jacalin biotin (Vector Laboratories)

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Vector Laboratories jacalin biotin

Colon epithelial monolayers with histological signatures for studying Enterococcus faecalis mucosal colonization. ( A ) Schematic depicting the experimental setup. Human colon organoids grown in Matrigel were split into single cells and seeded on top of collagen-coated Transwell membranes. Once the epithelial monolayer formed, the cells were differentiated at the air-liquid interface. Transwells were used on day 7 after seeding. ( B ) Immunostaining images of differentiated organoid monolayers with MUC2 protein (yellow), nuclei stained with DAPI (blue), and mucus labeled <t>using</t> <t>Jacalin-biotin</t> and streptavidin-Cy5 (magenta). ( C ) Colony-forming units (CFU/mL) analysis of Ef growth in mucus 6 h after inoculation. Both time points represent the colony-forming units of bacteria present in the Transwell in four independent biological replicates. The horizontal black lines mark the mean. ( D ) Maximum intensity projection of Jacalin-labeled mucus (magenta) and Ef WT expressing pDasherGFP (green). ( E ) Ef WT expressing pDasherGFP (green) in the same z-plane as the Jacalin-labeled mucus (magenta) and does not colocalize with the colonoid epithelium labeled with CellMask-DeepRed (red). ( F ) Ef colony volume calculated from confocal images represented in panel C . A minimum of 148 clusters was quantified from each image of four independent biological replicates.

Jacalin Biotin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/B-1155-5/Biotinylated+Jacalin/pmc13098192-3-0-2
Average 94 stars, based on 86 article reviews

jacalin biotin - by Bioz Stars, 2026-11

94/100 stars

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1) Product Images from "Adaptation of Enterococcus faecalis to intestinal mucus revealed by a human colonic organoid model"

Article Title: Adaptation of Enterococcus faecalis to intestinal mucus revealed by a human colonic organoid model

Journal: mSystems

doi: 10.1128/msystems.01304-25

Figure Legend Snippet: Colon epithelial monolayers with histological signatures for studying Enterococcus faecalis mucosal colonization. ( A ) Schematic depicting the experimental setup. Human colon organoids grown in Matrigel were split into single cells and seeded on top of collagen-coated Transwell membranes. Once the epithelial monolayer formed, the cells were differentiated at the air-liquid interface. Transwells were used on day 7 after seeding. ( B ) Immunostaining images of differentiated organoid monolayers with MUC2 protein (yellow), nuclei stained with DAPI (blue), and mucus labeled using Jacalin-biotin and streptavidin-Cy5 (magenta). ( C ) Colony-forming units (CFU/mL) analysis of Ef growth in mucus 6 h after inoculation. Both time points represent the colony-forming units of bacteria present in the Transwell in four independent biological replicates. The horizontal black lines mark the mean. ( D ) Maximum intensity projection of Jacalin-labeled mucus (magenta) and Ef WT expressing pDasherGFP (green). ( E ) Ef WT expressing pDasherGFP (green) in the same z-plane as the Jacalin-labeled mucus (magenta) and does not colocalize with the colonoid epithelium labeled with CellMask-DeepRed (red). ( F ) Ef colony volume calculated from confocal images represented in panel C . A minimum of 148 clusters was quantified from each image of four independent biological replicates.

Techniques Used: Immunostaining, Staining, Labeling, Bacteria, Expressing

$Glycosyltransferase BgsB in E. faecalis is essential for growth in colonic mucus. ( A ) Table of log2FC values calculated from the Tn-seq experiment for the glycosyltransferase bgsB and a biofilm-forming factor bph . ( B ) Representative images of Ef colonies in colonic mucus at 9 h of growth from three biological replicates. Mucus was labeled using Jacalin-biotin and streptavidin-Cy5. Wild-type Ef fluorescently expressed pDasher-GFP, and bgsB and bph mutants expressed tdTomato. ( C ) Colony-forming unit (CFU/mL) quantification of the deletion mutants grown in mucus compared with the wild-type Ef after 6 h of growth. Each dot is a biological replicate (at least three independent biological replicates for each mutant), and the horizontal black lines mark the mean. ( D ) Competition between WT (green) and mutant samples (orange) with over three biological replicates, where the WT vs bgsB::Tn is marked with a filled circle and WT vs Δ bph with an unfilled triangle. Volume ratio is calculated as a fraction of the mutant signal occupying the total (mutant + WT) volume in the image. Volume expansion is calculated as a ratio to the initial volume in the respective channel. Mean and standard deviation are shown. ( E ) The same mixed samples were grown in mucus in a flow chip shown in , under the flow of 5 µL/min of minimal medium ( n = 3). Arrows indicate the same place at the start and the end of the time-lapse. Statistics in panel C were calculated using one-way ANOVA with post hoc Dunnett’s multiple comparison test (*** P < 0.001).$
Figure Legend Snippet: Glycosyltransferase BgsB in E. faecalis is essential for growth in colonic mucus. ( A ) Table of log2FC values calculated from the Tn-seq experiment for the glycosyltransferase bgsB and a biofilm-forming factor bph . ( B ) Representative images of Ef colonies in colonic mucus at 9 h of growth from three biological replicates. Mucus was labeled using Jacalin-biotin and streptavidin-Cy5. Wild-type Ef fluorescently expressed pDasher-GFP, and bgsB and bph mutants expressed tdTomato. ( C ) Colony-forming unit (CFU/mL) quantification of the deletion mutants grown in mucus compared with the wild-type Ef after 6 h of growth. Each dot is a biological replicate (at least three independent biological replicates for each mutant), and the horizontal black lines mark the mean. ( D ) Competition between WT (green) and mutant samples (orange) with over three biological replicates, where the WT vs bgsB::Tn is marked with a filled circle and WT vs Δ bph with an unfilled triangle. Volume ratio is calculated as a fraction of the mutant signal occupying the total (mutant + WT) volume in the image. Volume expansion is calculated as a ratio to the initial volume in the respective channel. Mean and standard deviation are shown. ( E ) The same mixed samples were grown in mucus in a flow chip shown in , under the flow of 5 µL/min of minimal medium ( n = 3). Arrows indicate the same place at the start and the end of the time-lapse. Statistics in panel C were calculated using one-way ANOVA with post hoc Dunnett’s multiple comparison test (*** P < 0.001).

Techniques Used: Labeling, Mutagenesis, Standard Deviation, Comparison

Incubation:

Article Title: Epididymal epithelial cells facilitate NEU1 loading to modulate sperm α-2,6 sialylation, enhance maturation and motility
Article Snippet: Blocking was conducted with 3% BSA (BIOFROXX) in permeabilization buffer at RT for 1 h with rotation. .. Primary antibodies (anti-NEU1 [1:100, GenScript], anti-NEU3 [1:1000, GenScript], Biotinylated Maackia Amurensis Lectin I [anti-MAL-I, 1:50, Vector Labs], Biotinylated Maackia Amurensis Lectin II [anti-MAL-II, 1:50, Vector Labs], biotinylated Sambucus Nigra Lectin [anti-SNA, 1:50, Vector Labs], biotinylated Wheat Germ Agglutinin [anti-WGA, 1:50, Vector Labs], Peanut Agglutinin [anti-PNA, 1:100], anti-Jacalin [1:300, Vector Labs], Alexa Fluor 594 anti-biotinylated Phosphotyrosine [1:200, Biolegend]), anti-EGFR [1:300, ABclonal], anti-Phospho-EGFR [1:100, ABclonal], JC-1 [1:200, Beyotime], DCFH-DA [1:50, Beyotime] were incubated overnight at 4 °C. .. The next day, samples were centrifuged, washed with PBST, and incubated with secondary antibodies (PE Donkey anti-rabbit IgG [1:1000, Biolegend], FITC Streptavidin [1:500, Biolegend], DyLight 488 Streptavidin [1:500, Biolegend]) in permeabilization buffer at RT for 1 h. After PBST washes, samples were stained with DAPI (Beyotime) and analyzed using a CytoFLEX flow cytometer.

Article Title: Epididymal epithelial cells facilitate NEU1 loading to modulate sperm α-2,6 sialylation, enhance maturation and motility.
Article Snippet: Blocking was conducted with 3% BSA (BIOFROXX) in permeabilization buffer at RT for 1 h with rotation. .. Primary antibodies (anti-NEU1 [1:100, GenScript], anti-NEU3 [1:1000, GenScript], Biotinylated Maackia Amurensis Lectin I [anti-MAL-I, 1:50, Vector Labs], Biotinylated Maackia Amurensis Lectin II [anti-MAL-II, 1:50, Vector Labs], biotinylated Sambucus Nigra Lectin [anti-SNA, 1:50, Vector Labs], biotinylated Wheat Germ Agglutinin [anti-WGA, 1:50, Vector Labs], Peanut Agglutinin [antiPNA, 1:100], anti-Jacalin [1:300, Vector Labs], Alexa Fluor 594 anti-biotinylated Phosphotyrosine [1:200, Biolegend]), anti-EGFR [1:300, ABclonal], anti-Phospho-EGFR [1:100, ABclonal], JC-1 [1:200, Beyotime], DCFH-DA [1:50, Beyotime] were incubated overnight at 4 °C. .. The next day, samples were centrifuged, washed with PBST, and incubated with secondary antibodies (PE Donkey anti-rabbit IgG [1:1000, Biolegend], FITC Streptavidin [1:500, Biolegend], DyLight 488 Streptavidin [1:500, Biolegend]) in permeabilization buffer at RT for 1 h. After PBST washes, samples were stained with DAPI (Beyotime) and analyzed using a CytoFLEX flow cytometer.

Article Title: Glycoprotein G enables HSV-2 neuroinvasion and provides protection as a glycosylated vaccine antigen
Article Snippet: Lectin blot was performed by loading, 2 μg each of EXCT4-mgG-2, EXCT4-mgG-2(−N−O), EXCT4-mgG-2(-N), EXCT4-mgG-2(-O) and EXCT4-mgG-2(-SA) to a NuPAGE Bis-Tris 4-12% gel (Invitrogen) and separated using MOPS buffer (Invitrogen) at 200 V. The separated proteins were transferred to a polyvinylidene difluoride membrane (Immobilon-FL 0.45 μm, (Merck Millipore)) using the SemiDry Transblot SD (Bio-Rad Laboratories). .. Membranes were blocked with block buffer (2% BSA Factor V (Sigma-Aldrich) and 0.1% Tween-20 (VWR chemicals) in PBS (Medicago) followed by incubation with biotinylated lectins; 3 μg/mL Concavalin A (Con A) (Vector Laboratories), 3 μg/mL Jacalin (Vector Laboratories) or 5 μg/mL Maackia Amurensis Lectin II (MAL II, Vector Laboratories) diluted in PBS-BSA-T, at 4 °C for 16-20 hours. .. Next, membranes were washed three times with 0.1% Tween-20 in PBS (Medicago) followed by incubation with Streptavidin-alkaline phosphatase diluted 1:2000 (Southern Biotech) for 1 h at 20-22 °C.

Blocking Assay:

Staining:

Article Title: Layer- and region-specific distribution of chondroitinase-resistant, lectin-binding extracellular matrix structures in the mouse somatosensory cortex and hippocampus.
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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jacalin biotin (Vector Laboratories)

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1) Product Images from "Adaptation of Enterococcus faecalis to intestinal mucus revealed by a human colonic organoid model"

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